Course unit title Level of course unit Course unit code Type of course unit Semester of course unit Local credit ECTS credit Syllabus
PROTEIN PURIFICATION AND CHARACTERIZATION First cycle CEBK 523 1 8.00 8.00 Print
   
Description of course unit
Prerequisites and course requisities
Language of instruction Turkish
Coordinator Assistant Prof. Ahmet CUMAOĞLU
Lecturer(s) Assistant Prof. Ahmet CUMAOĞLU
Teaching assitant(s) none
Mode of delivery theoretical
Course objective Methods and approaches for isolation purification and characterization of the protein in Cell and tissue samples
Course description Structure of proteins, amino acids, isoelectric point, isolation solutions, centrifugation, ultrafiltration and other types of filtration, chromatographic methods and theoretical knowledge about the subject protein electrophoresis

Course contents
1 structures of amino acids,charges of amino acids according to pH, isoelectric point
2 Proteins and structures
3 Issues to be considered in isolation of proteins as temperature, pH, salt content and solvent
4 Centrifuging techniques and clarification
5 filtration
6 To concentrating proteins, dialysis, salting-out,salting in
7 Article presentation
8 Introduction to Chromatography
9 Affinity and ion exchange chromatography
10 Hydrophobic interaction and size exclusion chromatography
11 Article presentation
12 HPLC and Mass spectroscopy
13 SDS-PAGE
14 Western Blot
15
16
17
18
19
20

Learning outcomes of the course unit
1 to give information about purification and characterization of proteins from the cell or tissue
2
3
4
5
6
7
8
9
10

*Contribution level of the course unit to the key learning outcomes
1 Have the theoretical and conceptual knowledge necessary to understand the community
2 To understand and explain to social structure and social action
3 To understand the role of sociology in understanding social reality
4 Make sociological analysis using the basic sociological theories and concepts
5 Respect for different social groups and cultures
6 To make a social research with technical information
7 Critical thinking and analytical
8 To have awareness and sensitivity about social issues
9 Predict on sociological development
10 Accumulation to ensure that ethical issues concerning sociological research
11 Ability to understand and explain the differences between cultures
12 To use in business research, methods and techniques
13 Gain a interdisciplinary perspective
14 To have professional sensitivity and ethical responsibility
15 -
16 -
17 -
18 -
19 -
20 -
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
Number of stars refer to level of contribution from 1 (the least) to 5 (the most)

Planned learning activities, teaching methods and ECTS work load
  Quantity Time (hour) Quantity*Time (hour)
Lectures (face to face teaching) 14 3 42
Study hours out of classroom (study before and after the class) 14 3 42
Homework 5 5 25
Presentation / seminar 5 10 50
Quiz 0 0 0
Preparation for midterm exams 1 15 15
Midterm exams 1 2 2
Project (term paper) 0 0 0
Laboratuar 0 0 0
Field study 0 0 0
Preparation for final exam 1 20 20
Final exam 1 2 2
Research 0 0 0
Total work load     198
ECTS     8.00

Assessment methods and criteria
Evaluation during semester Quantity Percentage
Midterm exam 1 40
Quiz 0 0
Homework 0 0
Semester total   40
Contribution ratio of evaluation during semester to success   40
Contribution ratio of final exam to success   60
General total   100

Recommended and required reading
Textbook Protein Purification Guide, Aktif Publications
Additional references

Files related to the course unit