Erciyes University - Info Package

Course unit title	Level of course unit	Course unit code	Type of course unit	Semester of course unit	Local credit	ECTS credit	Syllabus
PROTEIN SEPERATION AND PURIFICATION METHODS	Third cycle	KIM 675		1	7.50	7.50	Print

Description of course unit

Prerequisites and course requisities	-
Language of instruction	Turkish
Coordinator	Assist. Prof. Dr. Nalan Özdemir
Lecturer(s)	Assist. Prof. Dr. Nalan Özdemir
Teaching assitant(s)	-
Mode of delivery	Face to face
Course objective	Understanding the properties of the proteins and principles of the protein purifications
Course description	Properties of biological products, pretreatment, enzymatic , chemical and physical cell fragmentation methods, isolation of the proteins, sources of proteins biological objects, protein stabilisation, qualitative and quantitative determination of protein, centrifuge and centrifuge applications, precipitation and applications of precipitation, fractional precipitation, chromatographic methods and chromatographic separations, gel filtration chromatography, ion exchange chromatography, affinity chromatography , paper chromatography, other chromatographic techniques, electrophoresis and electrophoretic methods, gel electrophoresis, SDS- PAGE, isoelectric focusing , two-dimensional (2D) electrophoresis.

Course contents

1	Properties of biological products
2	Pretreatment, enzymatic , chemical and physical cell fragmentation methods
3	Isolation of the proteins, sources of proteins biological objects
4	Protein stabilisation, qualitative and quantitative determination of protein
5	Centrifuge and centrifuge applications
6	Precipitation and applications of precipitation, fractional precipitation
7	Midterm exam
8	Chromatographic methods and chromatographic separations
9	Chromatographic methods and chromatographic separations, gel filtration chromatography, ion exchange chromatography
10	Chromatographic methods and chromatographic separations, affinity chromatography , paper chromatography, other chromatographic techniques
11	Electrophoresis and electrophoretic methods, gel electrophoresis
12	Electrophoresis and electrophoretic methods, SDS- PAGE
13	Electrophoresis and electrophoretic methods, isoelectric focusing
14	Two-dimensional ( 2D) electrophoresis
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Learning outcomes of the course unit

1	Understanding the properties of biological products
2	Understanding the isolation of the proteins, sources of proteins biological objects
3	Understanding the qualitative and quantitative determination of proteins
4	Understanding the centrifuge and centrifuge applications
5	Understanding the precipitation and applications of precipitation
6	Understanding the chromatographic methods and chromatographic separations
7	Understanding the electrophoresis and electrophoretic methods
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*Contribution level of the course unit to the key learning outcomes

1	Students learn concepts of the department, subject, methods and problems.
2	New and complex ideas is analyzed, synthesized and evaluated by the students.
3	They recognize new developments in the field of science.
4	They recognizes domestic and foreign sources and use effectively in their studies.
5	They understand the interdisciplinary interaction related to field of science.
6	They development original ideas to bring innovation to the field of science, methodology, practice and research.
7	They take part in national and international projects related to field of science or develop new projects.
8	They use information and communication technologies effectively in scientific studies related to field of science.
9	They know Arabic and Western languages at an advanced level and use.
10	They have scientific and professional ethics.
11	They contribute to the work of academic institutions and organizations.
12	They collaborate with relevant individuals and organizations about issues related to field of science and develop joint projects.
13	They share results that they reached in national and international academic platforms.
14	They help to develop a scientific, social, cultural, etc. strategy on issues related to their profession.
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Number of stars refer to level of contribution from 1 (the least) to 5 (the most)

Planned learning activities, teaching methods and ECTS work load

	Quantity	Time (hour)	Quantity*Time (hour)
Lectures (face to face teaching)	14	3	42
Study hours out of classroom (study before and after the class)	14	3	42
Homework	14	2	28
Presentation / seminar	5	2	10
Quiz	3	3	9
Preparation for midterm exams	1	3	3
Midterm exams	1	3	3
Project (term paper)	0	0	0
Laboratuar	0	0	0
Field study	0	0	0
Preparation for final exam	1	3	3
Final exam	1	3	3
Research	14	3	42
Total work load			185
ECTS			7.50

Assessment methods and criteria

Evaluation during semester	Quantity	Percentage
Midterm exam	2	40
Quiz	0	0
Homework	0	0
Semester total		40
Contribution ratio of evaluation during semester to success		40
Contribution ratio of final exam to success		60
General total		100

Recommended and required reading

Textbook
Additional references

Files related to the course unit