Course unit title Level of course unit Course unit code Type of course unit Semester of course unit Local credit ECTS credit Syllabus
PRINCIPLES OF BIOCHEMISTRY LABORATORY Second cycle CEBK 519 1 8.00 8.00 Print
   
Description of course unit
Prerequisites and course requisities None
Language of instruction Turkish
Coordinator Doç.Dr.Behzat Çimen
Lecturer(s) Doç.Dr.Behzat Çimen
Teaching assitant(s) None
Mode of delivery The emphasis of this course will be on theoretical and practical subject and methodological solve of laboratory analysis. The class meets for three lectures a week, each consisting of 50-minute sessions. In lectures, interactive training techniques and various audio-visual materials will be used.
Course objective The objective of this course is to teach the student methods and principles of laboratory analysis.
Course description Only postgraduate students are accepted for the course

Course contents
1 Safety in the laboratory
2 General laboratory procedures: Use of microscope, weighing, centrifugation, measurement and dispensing of liquids, claining, disinfection and sterilization, disposal of laboratory waste, quality assurance in the laboratory.
3 Water for laboratory use: Clean water, distilled water, demineralized water, buffered water.
4 Equipment: Essential laboratory instruments.
5 The densities of liquids and solids. Determination of chemical formula. Molar mass of volatile liquid. Properties of hydrate
6 EXAM
7 Classifications of chemical substances and reactions. Rates of chemical reactions. Determination of the equilibrium constant for a chemical reaction, Chemical kinetics
8 pH measurements-buffers and their properties.
9 Introduction to Principles of end-product analysis in biochemistry laboratory.
10 Principles of end-product analysis in biochemistry laboratory:Examples: Measurement principles of lipid peroxidations products and MDA, protein oxidations products and carbonyl groups, peroxynitrite end-product nitrotyrosine, DNA damage product hydroxy deoxy guanosine, etc…
11 Introduction to principles of experimental determination of kinetic analysis.
12 Principles of experimental determination of kinetic analysis: Examples: Some enzyme analysis
13 Principles of preparation of dilution series
14 Principles of stock solution, standard solution preparation and the standard curve drawing
15 FİNAL EXAMINATION
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Learning outcomes of the course unit
1 to teach the student methods and principles of laboratory analysis.
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*Contribution level of the course unit to the key learning outcomes
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Number of stars refer to level of contribution from 1 (the least) to 5 (the most)

Planned learning activities, teaching methods and ECTS work load
  Quantity Time (hour) Quantity*Time (hour)
Lectures (face to face teaching) 14 3 42
Study hours out of classroom (study before and after the class) 14 3 42
Homework 5 5 25
Presentation / seminar 5 10 50
Quiz 0 0 0
Preparation for midterm exams 1 15 15
Midterm exams 1 2 2
Project (term paper) 0 0 0
Laboratuar 0 0 0
Field study 0 0 0
Preparation for final exam 1 20 20
Final exam 1 2 2
Research 0 0 0
Total work load     198
ECTS     8.00

Assessment methods and criteria
Evaluation during semester Quantity Percentage
Midterm exam 1 20
Quiz 0 0
Homework 1 20
Semester total   40
Contribution ratio of evaluation during semester to success   40
Contribution ratio of final exam to success   60
General total   100

Recommended and required reading
Textbook John S Varcoe. Clinical Biochemistry: Techniques and Instrümentation. World Scientific Publishing Co. 2001. Emil J Slowinski, Wayne C. Wolsey, Robert C Rossi. Chemical Principles in the Laboratory. Tenth Edition. Books/Cole.USA.2009. Postma, J. M. Roberts, J. L. Hollenberg, J. L. Chemistry in the Laboratory. Fifth Edition. W. H. Freeman and Company, New York, 2000.
Additional references

Files related to the course unit