Erciyes University - Info Package

Course unit title	Level of course unit	Course unit code	Type of course unit	Semester of course unit	Local credit	ECTS credit	Syllabus
GENERAL MICROBIOLOGY LABORATORY	First cycle	BİY305		5	3.00	3.00	Print

Description of course unit

Prerequisites and course requisities	None
Language of instruction	Turkish
Coordinator	Assoc. Prof. Dr. Servet ÖZCAN
Lecturer(s)	Assoc. Prof. Dr. Servet ÖZCAN
Teaching assitant(s)	None
Mode of delivery	Face to Face; The course will be presented in two-50 min experiments per week. Students will be encouraged to participate in each experiment and the results of each experiment will be discussed with students.
Course objective	Laboratory emphasis is on current techniques used to cultivate and identify bacteria, and on standard techniques used in applications of microbiology.
Course description	Laboratory emphasis is on bacteriological media preparation and sterilization, transfer and colony selection, use of immersion-oil microscopy, Gram, endospore and capsule staining protocols, biochemical activities of carbon and nitrogen metabolism, IMVIC series, control of bacterial growth chemical and physical factors, determination of oxygen requirements, examination of inhibitory actions of heavy metals and biologically active compounds with agar diffusion test. Examination of floral specimen obtained from human body fluid.

Course contents

1	Introduction to microbiology laboratory and its equipment
2	Medium preparation and sterilization
3	Culture methods
4	Microscopy techniques and Gram staining
5	Endospor and capsule staining
6	Identification
7	Biochemical activity tests
8	Physical and chemical requirements of microorganisms
9	Effect of temperature
10	Effect of osmotic pressure and pH
11	Aerobic and anaerobic culture technique
12	Antibiograme tests
13	Microbial counting techniques
14	Throat and skin flora studies
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Learning outcomes of the course unit

1	Microbiology laboratory settings, hygiene rules of operation, sterile working techniques are learned
2	Will have the knowledge and the skills necessary to prepare microbiological media and learn how to make necessary calculations
3	Will learn the streaking techniques of cultivation for microorganisms
4	Will have the skills of staining methods used in diagnosis of bacteria
5	Effects of the environmental conditions (such as oxygen, acids, UV) on growth of microorganisms learned
6	Antibiotic resistance and sensitivity testing (such as disk diffusion and so on) are learned
7	Measuring the growth of microorganisms, growth curve techniques and counting the number of cells present in a given sample are learned
8	Cultivation of pathogenic bacteria from throat flora associated samples exercised
9	Hemolysis of blood agar test performed and colonies are distinguished and characterized
10	Student have the ability to fallow/discuss laboratory test results within the microbiology field

*Contribution level of the course unit to the key learning outcomes

1	have advanced theoretical and practical knowledge in the field of natural sciences that builds upon their secondary education and is supported by textbooks, course materials and other scientific resources presenting up-to-date information.
2	Make use of theoretical and practical knowledge acquired in the field of mathematics and natural science and chemistry.
3	The ability to have scientific and ethical values.
4	To solve unexpected problems in related applications of chemistry field.
5	The ability to plan and manage activities required for professional development.
6	Critically evaluate the accuracy and relevancy of knowledge and skills acquired; to define andassess learning needs; and to direct learning processes.
7	The ability to offer solutions to problems.
8	The ability of sharing their opinions or solutionoffers to the problems to specialists or non-specialists, supporting these with qualitative and quantitative data.
9	The ability to have enough competency in a foreign language to follow the literature in chemistry and communicate with their pers.
10	The ability to use computer software and communication and information technologies required in the field of chemistry competently and use theseto access scientific resources
11	The ability to comply with social, scientific and ethical values in the process of collecting, interpreting and using data and reporting the results in the field of chemistry
12	Awareness of the environmental protection and work/laboratory safety.
13	Have the skills towork in interdisciplinary subjects
14	To have skills to use modern devices required for the practices.
15	To have competency in keeping up with global innovations and developments in chemistry and in related fields.
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Number of stars refer to level of contribution from 1 (the least) to 5 (the most)

Planned learning activities, teaching methods and ECTS work load

	Quantity	Time (hour)	Quantity*Time (hour)
Lectures (face to face teaching)	14	2	28
Study hours out of classroom (study before and after the class)	14	2	28
Homework	5	2	10
Presentation / seminar	0	0	0
Quiz	0	0	0
Preparation for midterm exams	1	10	10
Midterm exams	1	2	2
Project (term paper)	0	0	0
Laboratuar	0	0	0
Field study	0	0	0
Preparation for final exam	1	12	12
Final exam	1	2	2
Research	1	5	5
Total work load			97
ECTS			4.00

Assessment methods and criteria

Evaluation during semester	Quantity	Percentage
Midterm exam	1	50
Quiz	1	30
Homework	1	20
Semester total		100
Contribution ratio of evaluation during semester to success		40
Contribution ratio of final exam to success		60
General total		100

Recommended and required reading

Textbook	1. Gerhardt, P., Murray, R. G. E., Costilow, P. N., Nester, E. W., Wood, W. A., Krieg, N. R., Phillips, G. B., Manuel of Methods for General Bacteriology, ASM, USA, 1981. 2. Temiz, A., General Microbiology Application & Techniques, 3. ed., Hatipoğlu Yayınevi, Ankara, 2000.
Additional references

Files related to the course unit

About CIP

About the Course

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