Erciyes University - Info Package

Course unit title	Level of course unit	Course unit code	Type of course unit	Semester of course unit	Local credit	ECTS credit	Syllabus
MOLECULER GENETICS RESEARCHING METHODS	Third cycle	GEN 607		1	8.00	8.00	Print

Description of course unit

Prerequisites and course requisities	Only doctorate students are accepted for the course
Language of instruction	Turkish
Coordinator	Prof.Dr. Yusuf ÖZKUL
Lecturer(s)	Prof.Dr. Yusuf ÖZKUL
Teaching assitant(s)	Research Assistant
Mode of delivery	practical and theorical
Course objective	The objective of this course is to teach the student the Moleculer Genetics Researching Methods
Course description	to teach the student the Moleculer Genetics Researching Methods

Course contents

1	DNA isolation (blood,tissue, amnion, paraphin block) , measurement of DNA,RNA and protein quantity,
2	Southern Blot, PCR, Gel Electrophoresis, Restriction Endonucleases,DNA sequencing,
3	SSCP,ASO,RFLP,ARMS,approaching to unknowing mutations, RNA isolation, Northen Blot, cDNA synthesis, RT PCR, protein isolation and Western Blot.
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Learning outcomes of the course unit

1	DNA isolation (blood,tissue, amnion, paraphin block) , measurement of DNA,RNA and protein quantity will be taught.
2	Southern Blot, PCR, Gel Electrophoresis, Restriction Endonucleases,DNA sequencing will be taught.
3	SSCP,ASO,RFLP,ARMS,approaching to unknowing mutations, RNA isolation, Northen Blot, cDNA synthesis, RT PCR, protein isolation and Western Blot will be taught.
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10

*Contribution level of the course unit to the key learning outcomes

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Number of stars refer to level of contribution from 1 (the least) to 5 (the most)

Planned learning activities, teaching methods and ECTS work load

	Quantity	Time (hour)	Quantity*Time (hour)
Lectures (face to face teaching)	14	2	28
Study hours out of classroom (study before and after the class)	26	3	78
Homework	0	0	0
Presentation / seminar	10	2	20
Quiz	0	0	0
Preparation for midterm exams	1	20	20
Midterm exams	1	0	0
Project (term paper)	0	0	0
Laboratuar	14	2	28
Field study	0	0	0
Preparation for final exam	1	20	20
Final exam	1	2	2
Research	0	0	0
Total work load			196
ECTS			8.00

Assessment methods and criteria

Evaluation during semester	Quantity	Percentage
Midterm exam	1	40
Quiz	0	0
Homework	0	0
Semester total		40
Contribution ratio of evaluation during semester to success		40
Contribution ratio of final exam to success		60
General total		100

Recommended and required reading

Textbook	1-N. Başaran,Tıbbi Genetik Ders Kitabı,1999 2-Connor,J.M,Ferguson-Smith,M.A.:Essential Medical Genetics.Blackwell Scientific Publications,Third Ed.,1991 3-Emery,A.E.H.,(Ed),Rimoin,D.L.:Principles and Practice of Medical Genetics.Third edition,Volume 1,Churchill Livingsto,London,
Additional references	4-Thompson&Thompson,Genetics in Medicine,6.th edition,2001

Files related to the course unit