Course unit title Level of course unit Course unit code Type of course unit Semester of course unit Local credit ECTS credit Syllabus
NUCLEIC ACID METHYLATION Second cycle TIB 516 1 4.00 4.00 Print
   
Description of course unit
Prerequisites and course requisities Having a lisance level of knowledge related to DNA structure and replication, RNA structure, synthesis, kinds and runctions,, protein structure and synthesis, and regulation of gen expression.
Language of instruction Turkish
Coordinator PROF. DR. NURHAN CÜCER
Lecturer(s) PROF. DR. NURHAN CÜCER
Teaching assitant(s) NONE
Mode of delivery Face to face presentation
Course objective Making students to have basic knovledge related to DNA methylation and its results in cells.
Course description Expressing master level knowledke on epigenetic mechanisms and especially DNA methylation

Course contents
1 Description of epigenetics and known epicenetic regulation mechanisma.
2 Forms of DNA, aspects and conditionsfort he formotion of these forms.. Z DNA, its aspects and role in gene regulation.
3 Histon acetilation, effects, protein systems have rol in histon acetylation. Remodeling of DNA
4 The neaning of Methylation. Protein, RNA and DNA methylation, enzymes playing role, comparison of procaryota and eucaryota. Relation with restriction endonucleases, e.t.c..
5 In human and other mammalia. And other species. DNA methylation, Methylation- demethylation, - hiper and -hipo methylation,, tissue specific heredity proportions and pattern.
6 CpG islands, methyl-Cytosine and resemblance to timine and effects. Comparison of the gene promoters regulated on methylation and other mechanisms
7 Results of changes in specific methylation pattern of species or tissue.
8 Description, origin, functions and laboratory use of rRestriction enzymes. Hpa II ve Msp I examples, and searching whole genom DNA methylation ,
9 Old and new methods: Comparison
10 Genomic imprinting, X chromatin, natural and unnatural, single gene methylations and their results.
11 Unnatural demetilation and hypermethylation and their association with disseases.
12 Usage of DNA hipomethylation in some disseases via hemoglobin gene family example..
13 Article discussion
14 General discussion
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Learning outcomes of the course unit
1 Role of methylation in gene activity- phenotype formation relation, , Drosophyla has not DNA methylation, there is only one allels expression of some genes for normal phenotype, will be known.
2 While choosing animal research, it will be known that DNAs of some organisma have not got methylatted.
3 Students will know, how to distinguish the genes regulated via methylation from other genes, looking their promoter sequences..
4 They might produce new ideas of diagnosis or treatment on DNA methylation mecanism
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*Contribution level of the course unit to the key learning outcomes
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Number of stars refer to level of contribution from 1 (the least) to 5 (the most)

Planned learning activities, teaching methods and ECTS work load
  Quantity Time (hour) Quantity*Time (hour)
Lectures (face to face teaching) 14 1 14
Study hours out of classroom (study before and after the class) 14 3 42
Homework 0 0 0
Presentation / seminar 1 10 10
Quiz 0 0 0
Preparation for midterm exams 0 0 0
Midterm exams 0 0 0
Project (term paper) 0 0 0
Laboratuar 0 0 0
Field study 0 0 0
Preparation for final exam 1 28 28
Final exam 1 1 1
Research 0 0 0
Total work load     95
ECTS     4.00

Assessment methods and criteria
Evaluation during semester Quantity Percentage
Midterm exam 0 0
Quiz 0 0
Homework 0 0
Semester total   0
Contribution ratio of evaluation during semester to success   40
Contribution ratio of final exam to success   60
General total   100

Recommended and required reading
Textbook NONE
Additional references NONE

Files related to the course unit