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Dersin Tanımı
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Basic concepts; structure of enzymes, bioenergetic; Enzyme kinetics; Michealis Menten, Lineweaver-Burk and Eadie-Hofstee equations, effects of temperature and pH; Kinetics of unireactant enzymes: Velocity versus substrate concentration curve and reaction order; Graphical determination of Km and Vmax ; Simple inhibition systems: Competitive, non-competitive and un-competitive inhibitions; Substrate inhibition; Rapid equilibrium partial and mixed type inhibition: Partial competitive and non-competitive inhibitions; Linear mixed-type inhibitions; Hyperbolic mixed type inhibiton; Allosterik enzymes. Enzyme activation. Immobilization, methods and applications, immobilized enzymes
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